Professor Di Chen and Professor Zhigang Wang research teams of SJTU published extraordinary research results of separation and detection technology of gastric cancer cell-derived exosomes based on an innovative microfluidic chip

Recently, Professor Di Chen and Professor Zhigang Wang’s research team jointly published a highly impactful paper “ExoSD chips for high-purity immunomagnetic separation and high-sensitivity detection of gastric cancer cell-derived exosomes” in the Biosensors and Bioelectronics (IF: 10.618), which is an internationally renowned journal dedicated to the research, design, development and application of biosensors and bioelectronics. It is also an interdisciplinary journal serving professionals who are interested in biosensors and bioelectronics used in innovative diagnostics and electronic devices.


Gastric cancer cell-derived exosomes as biomarkers have an extremely important application potential to the non-invasive detection of early-stage gastric cancer. However, the small size of exosomes (30–150 nm) results in huge challenges in separating and detecting them from complex media (e.g., plasma, urine, saliva, and cell culture supernatant). A highly integrated exosome separation and detection (ExoSD) chip is proposed by teams of Professor Chen and Professor Wang for immunomagnetic separation of exosomes from cell culture supernatant in a manner of continuous flow, leading to immunofluorescence detection of gastric cancer cell-derived exosomes with high sensitivity. The proposed ExoSD chip has been successfully microfabricated, demonstrating as a cutting-edge platform for exosome separation and detection. It can be served as a versatile platform to be adapted to the applications of separation and detection of other cell type-derived exosomes or cells.

The ExoSD chip has achieved a high exosome recovery rate (>80%) and purity rate (>83%) at an injection speed of 80 μL/min.


Furthermore, experimental results based on clinical serum samples of patients with gastric cancer (stages I and II) showed that the detection rate of ExoSD chips reached as high as 70%.


Master student Zixian Yu and Assistant Researcher Dr. Shujing Lin are major contributors of this paper. The corresponding authors are Professor Di Chen, Assistant Researcher Dr. Shujing Lin and Professor Zhigang Wang.

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[ 2021-09-15 ]